AIM-HIV PROJECT------------------------Instituto Salud Carlos III





Protective effect of vaginal application of neutralizing and nonneutralizing inhibitory antibodies against vaginal SHIV challenge in macaques. Moog C, Dereuddre-Bosquet N, Teillaud JL, Biedma ME, Holl V, Van Ham G, Heyndrickx L, Van Dorsselaer A, Katinger D, Vcelar B, Zolla-Pazner S, Mangeot I, Kelly C, Shattock RJ, Le Grand R. Mucosal Immunol. 2014 Jan;7(1):46-56.

Electroporation-mediated intradermal delivery of DNA vaccines in nonhuman primates. Adam L, Le Grand R, Martinon F. Methods Mol Biol. 2014;1121:309-13.

Plasmacytoid dendritic cell dynamics tune interferon-alfa production in SIV-infected cynomolgus macaques. Bruel T, Dupuy S, Démoulins T, Rogez-Kreuz C, Dutrieux J, Corneau A, Cosma A, Cheynier R, Dereuddre-Bosquet N, Le Grand R, Vaslin B. PLoS Pathog. 2014 Jan 30;10(1):e1003915. 

Semen CD4+ T cells and macrophages are productively infected at all stages of SIV infection in macaques. Bernard-Stoecklin S, Gommet C, Corneau AB, Guenounou S, Torres C, Dejucq-Rainsford N, Cosma A, Dereuddre-Bosquet N, Le Grand R. PLoS Pathog. 2013 Dec;9(12):e1003810.

Note on the formulation of thermosensitive and mucoadhesive vaginal hydrogels containing the miniCD4 M48U1 as anti-HIV-1 microbicideBouchemal K, Frelichowska J, Martin L, Lievin-Le Moal V, Le Grand R, Dereuddre-Bosquet N, Djabourov M, Aka-Any-Grah A, Koffi A, Ponchel G. Int J Pharm. 2013 Oct 1;454(2):649-52.

MiniCD4 microbicide prevents HIV infection of human mucosal explants and vaginal transmission of SHIV(162P3) in cynomolgus macaquesDereuddre-Bosquet N, Morellato-Castillo L, Brouwers J, Augustijns P, Bouchemal K, Ponchel G, Ramos OH, Herrera C, Stefanidou M, Shattock R, Heyndrickx L, Vanham G, Kessler P, Le Grand R, Martin L. PLoS Pathog. 2012;8(12):e1003071.

Infection of macaques after vaginal exposure to cell-associated simian immunodeficiency virusSallé B, Brochard P, Bourry O, Mannioui A, Andrieu T, Prevot S, Dejucq-Rainsford N, Dereuddre-Bosquet N, Le Grand R. J Infect Dis. 2010 Aug 15;202(3):337-44.

Assessment of mucosal immunity to HIV-1Jespers V, Harandi AM, Hinkula J, Medaglini D, Le Grand R, Stahl-Hennig C, Bogers W, El Habib R, Wegmann F, Fraser C, Cranage M, Shattock RJ, Spetz AL. Expert Rev Vaccines. 2010 Apr;9(4):381-94. Review

Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaquesMannioui A, Bourry O, Sellier P, Delache B, Brochard P, Andrieu T, Vaslin B, Karlsson I, Roques P, Le Grand R. Retrovirology. 2009 Nov 23;6:106.

Prevention of vaginal simian immunodeficiency virus transmission in macaques by postexposure prophylaxis with zidovudine, lamivudine and indinavirBourry O, Brochard P, Souquiere S, Makuwa M, Calvo J, Dereudre-Bosquet N, Martinon F, Benech H, Kazanji M, Le Grand R. AIDS. 2009 Feb 20;23(4):447-54.

Differential expression levels of MRP1, MRP4, and MRP5 in response to human immunodeficiency virus infection in human macrophagesJorajuria S, Dereuddre-Bosquet N, Naissant-Storck K, Dormont D, Clayette P. Antimicrob Agents Chemother. 2004 May;48(5):1889-91.

Kinetics of lymphocyte proliferation during primary immune response in macaques infected with pathogenic simian immunodeficiency virus SIVmac251: preliminary report of the effect of early antiviral therapyBenlhassan-Chahour K, Penit C, Dioszeghy V, Vasseur F, Janvier G, Rivière Y, Dereuddre-Bosquet N, Dormont D, Le Grand R, Vaslin B. J Virol. 2003 Dec;77(23):12479-93.




Determination of HIV tropism and its use in the clinical practice. Pérez-Olmeda M, Alcami J. Expert Rev Anti Infect Ther. 2013 Dec;11(12):1291-302. 

Structure-based design of an RNA-binding p-terphenylene scaffold that inhibits HIV-1 Rev protein function. González-Bulnes L, Ibáñez I, Bedoya LM, Beltrán M, Catalán S, Alcamí J, Fustero S, Gallego J. Angew Chem Int Ed Engl. 2013 Dec 9;52(50):13405-9. 

Transcription elongation regulator 1 (TCERG1) regulates competent RNA polymerase II-mediated elongation of HIV-1 transcription and facilitates efficient viral replication. Coiras M, Montes M, Montanuy I, López-Huertas MR, Mateos E, Le Sommer C, Garcia-Blanco MA, Hernández-Munain C, Alcamí J, Suñé C. Retrovirology. 2013 Oct 28;10(1):124.

Resistance to the most recent protease and non-nucleoside reverse transcriptase inhibitors across HIV-1 non-B subtypes. Anta L, Blanco JL, Llibre JM, García F, Pérez-Elías MJ, Aguilera A, Pérez-Romero P, Caballero E, Vidal C, Cañizares A, Gutiérrez F, Dalmau D, Iribarren JA, Soriano V, de Mendoza C; Drug Resistance Platform of the Spanish AIDS Research Network. J Antimicrob Chemother. 2013 Sep;68(9):1994-2002. 

HIV-1 exploits CCR5 conformational heterogeneity to escape inhibition by chemokines. Colin P, Bénureau Y, Staropoli I, Wang Y, Gonzalez N, Alcami J, Hartley O, Brelot A, Arenzana-Seisdedos F, Lagane B. Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9475-80. 

Immune activation promotes evolutionary conservation of T-cell epitopes in HIV-1. Sanjuán R, Nebot MR, Peris JB, Alcamí J. PLoS Biol. 2013;11(4):e1001523.

Use of RT-defective HIV virions: new tool to evaluate specific response in chronic asymptomatic HIV-infected individuals. Guardo AC, Alvarez-Fernández C, Arberas H, García-Pérez J, García F, Bargalló ME, Maleno MJ, Gatell JM, Mothe B, Alcami J, Sánchez-Palomino S, Plana M. PLoS One. 2013;8(3):e58927. 

The presence of HIV-1 Tat protein second exon delays fas protein-mediated apoptosis in CD4+ T lymphocytes: a potential mechanism for persistent viral production. López-Huertas MR, Mateos E, Sánchez Del Cojo M, Gómez-Esquer F, Díaz-Gil G, Rodríguez-Mora S, López JA, Calvo E, López-Campos G, Alcamí J, Coiras M. J Biol Chem. 2013 Mar 15;288(11):7626-44.

IL28B single-nucleotide polymorphism rs12979860 is associated with spontaneous HIV control in white subjects. Machmach K, Abad-Molina C, Romero-Sánchez MC, Abad MA, Ferrando-Martínez S, Genebat M, Pulido I, Viciana P, González-Escribano MF, Leal M, Ruiz-Mateos E; HIV Controllers Consortium of the AIDS Spanish Network. J Infect Dis. 2013 Feb 15;207(4):651-5. 

Rilpivirine resistance mutations in HIV patients failing non-nucleoside reverse transcriptase inhibitor-based therapies. Anta L, Llibre JM, Poveda E, Blanco JL, Alvarez M, Pérez-Elías MJ, Aguilera A, Caballero E, Soriano V, de Mendoza C; Resistance Platform of the Spanish AIDS Research Network. AIDS. 2013 Jan 2;27(1):81-5.

Olean-18-ene triterpenoids from Celastraceae species inhibit HIV replication targeting NF-kB and Sp1 dependent transcription. Osorio AA, Muñóz A, Torres-Romero D, Bedoya LM, Perestelo NR, Jiménez IA, Alcamí J, Bazzocchi IL. Eur J Med Chem. 2012 Jun;52:295-303. 

International network for comparison of HIV neutralization assays: the NeutNet report II. Heyndrickx L, Heath A, Sheik-Khalil E, Alcami J, Bongertz V, Jansson M, Malnati M, Montefiori D, Moog C, Morris L, Osmanov S, Polonis V, Ramaswamy M, Sattentau Q, Tolazzi M, Schuitemaker H, Willems B, Wrin T, Fenyö EM, Scarlatti G. PLoS One. 2012;7(5):e36438.

Protein kinase Ctheta is a specific target for inhibition of the HIV type 1 replication in CD4+ T lymphocytes. López-Huertas MR, Mateos E, Díaz-Gil G, Gómez-Esquer F, Sánchez del Cojo M, Alcamí J, Coiras M. J Biol Chem. 2011 Aug 5;286(31):27363-77. 

Quinoline-based compounds as modulators of HIV transcription through NF-kappaB and Sp1 inhibition. Bedoya LM, Abad MJ, Calonge E, Saavedra LA, Gutierrez C M, Kouznetsov VV, Alcami J, Bermejo P. Antiviral Res. 2010 Sep;87(3):338-44.

Modifications in host cell cytoskeleton structure and function mediated by intracellular HIV-1 Tat protein are greatly dependent on the second coding exonLópez-Huertas MR, Callejas S, Abia D, Mateos E, Dopazo A, Alcamí J, Coiras M. Nucleic Acids Res. 2010 Jun;38(10):3287-307.

SDF-1/CXCL12 production by mature dendritic cells inhibits the propagation of X4-tropic HIV-1 isolates at the dendritic cell-T-cell infectious synapseGonzález N, Bermejo M, Calonge E, Jolly C, Arenzana-Seisdedos F, Pablos JL, Sattentau QJ, Alcamí J. J Virol. 2010 May;84(9):4341-51.

Gold nanoparticles capped with sulfate-ended ligands as anti-HIV agentsDi Gianvincenzo P, Marradi M, Martínez-Avila OM, Bedoya LM, Alcamí J, Penadés S. Bioorg Med Chem Lett. 2010 May 1;20(9):2718-21.


HIV-1 isolation from infected peripheral blood mononuclear cells. Dispinseri S, Saba E, Vicenzi E, Kootstra NA, Schuitemaker H, Scarlatti G. Methods Mol Biol. 2014;1087:187-96.

Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycleSaba E, Origoni M, Taccagni G, Ferrari D, Doglioni C, Nava A, Lisco A, Grivel JC, Margolis L, Poli G. Mucosal Immunol. 2013 Nov;6(6):1081-90.

Impaired CD4+ T-cell restoration in the small versus large intestine of HIV-1-positive South Africans receiving combination antiretroviral therapy. Cassol E, Malfeld S, Mahasha P, Bond R, Slavik T, Seebregts C, Poli G, Cassol S, van der Merwe SW, Rossouw T. J Infect Dis. 2013 Oct 1;208(7):1113-22.

Identification of TRIM22 single nucleotide polymorphisms associated with loss of inhibition of HIV-1 transcription and advanced HIV-1 disease. Ghezzi S, Galli L, Kajaste-Rudnitski A, Turrini F, Marelli S, Toniolo D, Casoli C, Riva A, Poli G, Castagna A, Vicenzi E. AIDS. 2013 Sep 24;27(15):2335-44.

Cell-to-cell vs. cell-free HIV-1 transmission from macrophages to CD4+ T lymphocytes: lessons from the virology textbook. Poli G. AIDS. 2013 Sep 10;27(14):2307-8. 

Association study of common genetic variants and HIV-1 acquisition in 6,300 infected cases and 7,200 controls. McLaren PJ, Coulonges C, Ripke S, van den Berg L, Buchbinder S, Carrington M, Cossarizza A, Dalmau J, Deeks SG, Delaneau O, De Luca A, Goedert JJ, Haas D, Herbeck JT, Kathiresan S, Kirk GD, Lambotte O, Luo M, Mallal S, van Manen D, Martinez-Picado J, Meyer L, Miro JM, Mullins JI, Obel N, O'Brien SJ, Pereyra F, Plummer FA, Poli G, Qi Y, Rucart P, Sandhu MS, Shea PR, Schuitemaker H, Theodorou I, Vannberg F, Veldink J, Walker BD, Weintrob A, Winkler CA, Wolinsky S, Telenti A, Goldstein DB, de Bakker PI, Zagury JF, Fellay J. PLoS Pathog. 2013;9(7):e1003515. 

HIV-1 infected lymphoid organs upregulate expression and release of the cleaved form of uPAR that modulates chemotaxis and virus expression. Nebuloni M, Zawada L, Ferri A, Tosoni A, Zerbi P, Resnati M, Poli G, Genovese L, Alfano M. PLoS One. 2013 Jul 29;8(7):e70606. 

M1 polarization of human monocyte-derived macrophages restricts pre and postintegration steps of HIV-1 replication. Cassetta L, Kajaste-Rudnitski A, Coradin T, Saba E, Della Chiara G, Barbagallo M, Graziano F, Alfano M, Cassol E, Vicenzi E, Poli G. AIDS. 2013 Jul 31;27(12):1847-56.

Macrophage polarization at the crossroad between HIV-1 infection and cancer development. Alfano M, Graziano F, Genovese L, Poli G. Arterioscler Thromb Vasc Biol. 2013 Jun;33(6):1145-52. Review.

The puzzling role of CXCR4 in human immunodeficiency virus infection. Vicenzi E, Liò P, Poli G. Theranostics. 2013;3(1):18-25. Review.

Novel factors interfering with human immunodeficiency virus-type 1 replication in vivo and in vitro. Vicenzi E, Poli G. Tissue Antigens. 2013 Feb;81(2):61-71. Review.

Dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin mediates HIV-1 infection of and transmission by M2a-polarized macrophages in vitro. Cassol E, Cassetta L, Rizzi C, Gabuzda D, Alfano M, Poli G. AIDS. 2013 Mar 13;27(5):707-16.

HIV-1 envelope-dependent restriction of CXCR4-using viruses in child but not adult untransformed CD4+ T-lymphocyte linesMariani SA, Brigida I, Kajaste-Rudnitski A, Ghezzi S, Rocchi A, Plebani A, Vicenzi E, Aiuti A, Poli G. Blood. 2012 Mar 1;119(9):2013-23.

TRIM22 inhibits HIV-1 transcription independently of its E3 ubiquitin ligase activity, Tat, and NF-kappaB-responsive long terminal repeat elementsKajaste-Rudnitski A, Marelli SS, Pultrone C, Pertel T, Uchil PD, Mechti N, Mothes W, Poli G, Luban J, Vicenzi E. J Virol. 2011 May;85(10):5183-96.

Sulfated K5 Escherichia coli polysaccharide derivatives: A novel class of candidate antiviral microbicidesRusnati M, Vicenzi E, Donalisio M, Oreste P, Landolfo S, Lembo D. Pharmacol Ther. 2009 Sep;123(3):310-22. Review.


NEWS 01  


Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of menstrual cycle. Read more.    




10/01/2014. The partners of the project and the Scientific Advisory Board attended the Progress Meeting of the project in Madrid.  



October 7-10, 2013. ISCIII and SEPROX presented an oral communication and a poster in the AIDS Vaccine Conference. Read more.


Instituto de Salud Carlos III. 2013. All rights reserved

This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 305938.

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